Tag
Part:BBa_K4365011:Design
Designed by: Svetlana Iarovenko Group: iGEM22_TU_Dresden (2022-09-29)
Signal peptide of HCF-1 from Cladosporium fulvum
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimized for yeast.
Source
The sequences of the hydrophobic signal peptide was collected from literature [1] and was extracted via analysis of their sequence using the SignalP - 5.0 signal peptide predictor tool [2].
References
- ↑ G. C. Segers, W. Hamada, R. P. Oliver, P. D. Spanu (1999) Isolation and characterisation of five different hydrophobin-encoding cDNAs from the fungal tomato pathogen Cladosporium fulvum, Molecular and General Genetics MGG volume 261, pages644–652 https://doi.org/10.1007/s004380050007
- ↑ José Juan Almagro Armenteros et al. (2019) SignalP 5.0 improves signal peptide predictions using deep neural networks Nature Biotechnology, 37, 420-423, doi: 10.1038/s41587-019-0036-z