Part:BBa_K4438905
crp_trigger_2_phi29
crp_trigger_3_phi29 (BBa_K4438905) is a single-stranded DNA having 34 nucleotides. Figure 1A) shows the secondary structure and minimum free energy. Its 3’ end has a few bases complementary to the part Aptamer_crp (BBa_K4438900). At the 5’ end, there are few bases of broccoli cDNA. The middle region has an ssDNA antisense T7 promoter sequence.
Usage and Biology
The part crp_trigger_3_phi29 (BBa_K4438905) binds to the part Aptamer_crp (BBa_K4438900) due to complementarity. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. CRP binds with Aptamer_crp (BBa_K4438900) and displaces the crp_trigger_3_phi29 (BBa_K4438905) [1]. This part has complete complementarity with part crp_Target_3(BBa_K4438906). Phi 29 DNA extension polymerase extends the template strand and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. Different levels of CRP can be detected using all these three parts.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Liu, Z., Luo, D., Ren, F., Ran, F., Chen, W., Zhang, B., ... & Chen, Q. (2019). Ultrasensitive fluorescent aptasensor for CRP detection based on the RNase H assisted DNA recycling signal amplification strategy. RSC advances, 9(21), 11960-11967.
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