Part:BBa_K4479000
F3 LAMP Primer for Oak Wilt V1
Loop-mediated Isothermal Amplification is a cheap and quick method to amplify a desired DNA target. LAMP uses four to six primers; F3, B3, FIP (Forward Inner Primer), BIP (Backward Inner Primer), and the optional LF (Loop Forward) and LB (Loop Backward) primers. We are using the four primer system that recognises six regions on our target DNA and they will form dumbbell-shaped amplicons. Our lamp primers run at 71°C and they are deactivated at 95°C. The primers were designed with the Primer Explorer software, then ranked for competency using a computer model. This is the F3 Primer of our first set of LAMP primers designed for the MCM7 region of Bretziella fagacearum (MG270170.1).
Characterization
LAMP on qPCR
A qPCR machine was used to measure the amplification of our LAMP master mix.
| Conc. (ng/uL) | Time (min) |
|---|---|
| 10 | 32.84 ± 2.86 |
| 5 | 40.19 ± 7.78 |
| 1 | 32.79 ± 2.95 |
| 0.5 | 31.98 ± 3.50 |
| 0.1 | 29.22 ± 8.50 |
| Average | 35 |
| None |