Primer

Part:BBa_K4361113

Designed by: Lars van den Biggelaar   Group: iGEM22_TUDelft   (2022-09-08)
Revision as of 13:40, 12 October 2022 by RobinKuijpers (Talk | contribs)


Blc operator SDM primer R

A site-directed mutagenesis primer, to be used in conjunction with the mutation-introducingforward primer Part:BBa_K4361112. Primers can be used in a PCR reaction with the Part: Part:BBa_K4361115 part as DNA template. A deletion will be introduced in the blc operator sequence, resulting in the altered sequence as described in Part: Part:BBa_K4361114.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 21
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 21
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 21
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None