Device

Part:BBa_K4206001

Designed by: Kazuma Tsurumaki   Group: iGEM22_Qdai   (2022-10-02)
Revision as of 13:37, 12 October 2022 by Darjeeling (Talk | contribs)


poxB-aatA, acetate production

This part is placed downstream of the lac promoter of a plasmid. poxB, acetate production enzyme, is induced by IPTG. aatA, coding sequence of transporter of acetate, expresses on downstream of constitutive promoter. Acetate is transported out of the cell and acetate synthesis is promoted for more efficient acetate production

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1870
    Illegal NheI site found at 1893
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 352
    Illegal SapI.rc site found at 1600


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Categories
Parameters
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