Coding

Part:BBa_K4291007

Designed by: Zhang Chenchen   Group: iGEM22_Canton_HS   (2022-08-12)
Revision as of 13:35, 12 October 2022 by Kimmy2020 (Talk | contribs)


gldh

gldh

The tnaC regulatory gene from the tna operon of E. coli controls the transcription of its own operon through an attenuation mechanism relying on the accumulation of arrested ribosomes during inhibition of its own translation termination. In this project, the expression of tnaC was regulated by tnaCAB operon in the pTrc99K vector. This mechanism requires ribosomal arrest induced by the regulatory nascent TnaC peptide in response to free L-tryptophan (L-Trp).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 403
    Illegal XhoI site found at 1174
    Illegal XhoI site found at 1216
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 589
    Illegal AgeI site found at 1093
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 775
    Illegal BsaI site found at 1024


[edit]
Categories
Parameters
None