Composite

Part:BBa_K4238005

Designed by: Keita Hoshino   Group: iGEM22_UTokyo   (2022-10-09)
Revision as of 13:34, 12 October 2022 by Asako (Talk | contribs)


C120-trucCYC1p+hbax+ADH1terminator
This Part functions as a blue light-sensitive kill switch when transformed into yeast with BBa_K4238003.
The C120-trucCYC1promoter causes dimeric EL222 to bind to the C120 sequence under blue light, allowing transcription of the downstream sequence. Therefore, hBax is expressed only when the yeast gets exposed to blue light, leading to yeast death. The apoptosis-inducible human Bax gene (BBa_K2365048) was used as the kill switch. hBax has been shown to be able to induce apoptosis in yeast. This kill switch is also expected to be utilized in the field of biosecurity, as apoptosis induces the thin cleavage of genome sequences. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 104
    Illegal SpeI site found at 79
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 86
    Illegal SpeI site found at 79
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 740
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 104
    Illegal SpeI site found at 79
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 104
    Illegal SpeI site found at 79
  • 1000
    COMPATIBLE WITH RFC[1000]


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