Composite

Part:BBa_K4238004

Designed by: Keita Hoshino   Group: iGEM22_UTokyo   (2022-10-09)
Revision as of 13:33, 12 October 2022 by Asako (Talk | contribs)


C120-trucCYC1p+mCherry+ADH1t
When transformed into yeast together with BBa_K4238003, this part causes the yeast to produce red fluorescence under blue light. The dimeric EL222 binds to the C120 sequence in the C120-trucCYC1promoter under blue light, allowing transcription of the downstream sequence. The expression can be quantified with the fluorescence of mCherry. A similar part was created in which a kill switch (BBa_K4238005) deriving from the human Bax gene is used instead of mCherry.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 104
    Illegal SpeI site found at 79
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 86
    Illegal SpeI site found at 79
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 104
    Illegal SpeI site found at 79
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 104
    Illegal SpeI site found at 79
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None