Composite

Part:BBa_K4238006

Designed by: Keita Hoshino   Group: iGEM22_UTokyo   (2022-10-09)
Revision as of 13:20, 12 October 2022 by Asako (Talk | contribs)


ADH1p+Cre+CYC1t

Cre is an enzyme that performs site-specific recombination and can be used to delete a part of sequences irreversibly.

Cre has the enzymatic activity of circularly excising the region flanked by recognition sequences called loxP. A constitutive promoter ADH1 and CYC1 terminator are used so that this part functions in yeast.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 919
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None