Regulatory

Part:BBa_K4202025:Design

Designed by: Zhijian Yan   Group: iGEM22_ZJU-China   (2022-10-03)
Revision as of 13:20, 12 October 2022 by Simplest (Talk | contribs) (→‎Design Notes)

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Pveg-1X leader RNA for Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Recombination of Pveg with the leader RNA of PsacB was designed to enhance promoter activity while reducing leaky expression.

The spacer sequence is desigened using online tools(http://crispr.dfci.harvard.edu/SSC/).

Source

The original sequence of leader RNA is obtained from subtiwiki (http://subtiwiki.uni-goettingen.de/v4/gene?id=AAA944BE7F01CE90F7730EECA16F3E4ED77D165A). The sequence of leader RNA is obtained from genomic DNA of Bacillus subtilis through PCR.The original sequence of Pveg is obtained from BBa_K1364008(https://parts.igem.org/Part:BBa_K1364008).

References