Part:BBa_K4129133:Design

The fungal synthetic expression system with FunsTF70 and 6xLexO-Pmin

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 4186
  Illegal XbaI site found at 6099
  Illegal PstI site found at 141
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 911
  Illegal NheI site found at 2986
  Illegal PstI site found at 141
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 145
  Illegal BglII site found at 3173
  Illegal BamHI site found at 2920
  Illegal BamHI site found at 5380
  Illegal XhoI site found at 701
  Illegal XhoI site found at 1083
  Illegal XhoI site found at 4050
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 4186
  Illegal XbaI site found at 6099
  Illegal PstI site found at 141
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 4186
  Illegal XbaI site found at 6099
  Illegal PstI site found at 141
  Illegal NgoMIV site found at 3078
  Illegal NgoMIV site found at 6016
  Illegal AgeI site found at 1290
  Illegal AgeI site found at 1438
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1117
  Illegal BsaI.rc site found at 5730
  Illegal SapI site found at 323
  

Design Notes

The assembly was standardised to assemble the cassettes in bulk.

Source

The individual parts were either salvaged from in-house plasmids or de novo synthesised:

BBa_K4129024 was salvaged from a in-house plasmid

FunsTF70 assembled from FunsTF05(de novo synthesised from IDT) and HbaR16(de novo synthesised from twist)

BBa_K4129115 was salvaged from a in-house plasmid

BBa_J06504 salvaged from BBa_K3046004

BBa_K3669007 was salvaged from a in-house plasmid

BBa_K678036 was salvaged from a in-house plasmid