Part:BBa_K4344020

HSV-UL19 AA 816:1148-6xHis

UL19 gene, coding for the capsid protein (VP5) of herpes simplex virus with additional His-tag for purification

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 301
  Illegal NgoMIV site found at 448
  
• 1000
  COMPATIBLE WITH RFC[1000]

The sequence of UL19 encoding for HSV-1 major capsid protein was extracted from the complete https://www.ncbi.nlm.nih.gov/nuccore/KF498959.1 human herpesvirus 1 genome. The sequence was human codon optimised using https://www.benchling.com/ Benchling. The sequence was screened for published antibody binding sites and functional structure elements using the https://www.uniprot.org/ UniProt database. We identified the amino acids 862 to 880 in UL19 as a published antibody binding site (Han et al., 2019). Since it is a published antibody site, we anticipate that the corresponding DNA sequence is highly conserved in the HSV genome. Therefore, we chose a sequence for cloning and expression including this structure element. For UL19 bases 2446 to 3444 were selected resulting in a fragment of 999 bp in size. The fragment was further modified to mask unwanted restriction sites and ease primer design while keeping the amino acid sequence unchanged. UL19 was modified at the 16-18TCC>AGT and 21G>A. A His-tag (5’-CATCACCATCACCATCAC-3’) and a TAA stop codon were added at the 3’ sequence end.