Coding

Part:BBa_K4169016

Designed by: Jie Min   Group: iGEM22_HZAU-China   (2022-09-30)
Revision as of 13:17, 12 October 2022 by Lloheha (Talk | contribs) (→‎Metabolic Pathway)


produce DMADH

After expressing, it'll produce dimethylamine dehydrogenase (DMADH). The enzyme DMADH is an iron–sulfur flavoprotein which catalyses the oxidative demethylation of dimethylamine (DMA) to methylamine and formaldehyde:
(CH3)2N + H2O → CH3NH + CH2O +2H+ + 2e-.

Metabolic Pathway

This enzyme is a complex iron-sulfur flavoprotein that transfers electrons to the soluble flavoprotein known as electron transferring flavoprotein[1]. It couldn't work extracellular isolated.


Figure 1.Pathways for dimethylamine metabolism in bacteria.


Protein Molecular Structures

Dimethylamine dehydrogenase(DMADH) exist as dimers.


Figure 2.Protein molecular structures of dimethylamine dehydrogenase.


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 897
    Illegal AgeI site found at 1291
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None