Plasmid_Backbone

Part:BBa_K4361107

Designed by: Lars van den Biggelaar   Group: iGEM22_TUDelft   (2022-09-07)
Revision as of 13:16, 12 October 2022 by RobinKuijpers (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pSB1C3, single letter prefix and suffix

This Part shows the linearized DNA sequence of the plasmid Part:pSB1C3 after digestion with EcoRI and PstI, allowing for inserts of BioBricks that have been digested with the same restriction enzymes. Gene can be inserted upfront a T7 promoter, this allows expression in presence of the T7 RNA polymerase. CmR gene ensures the resistance of chloramphenicol.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XhoI site found at 1013
    Illegal XhoI site found at 1905
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None