Coding

Part:BBa_K4390016

Designed by: Maarten van den Ancker, Serafina Soehianto   Group: iGEM22_Edinburgh-UHAS_Ghana   (2022-08-07)
Revision as of 13:11, 12 October 2022 by Dianajorge (Talk | contribs)


6xHis :: TEV protease cleavage site

This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.

This part is used as a removable His tag for protein purification. This sequence is for use in JUMP assembly and may be incompatible with non-Type IIS assembly techniques, as the fusion site regenerates some nucleotides.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None