Regulatory

Part:BBa_K4344002

Designed by: Marcel Pott   Group: iGEM22_Heidelberg   (2022-09-29)
Revision as of 13:06, 12 October 2022 by Leuntor (Talk | contribs)


CMV promoter enhancer

Forms together with BBa_K4344003 and BBa_K4344004 the CMV promoter for constitutive expression for use in mammalian cells. It was used with the plasmid backbones BBa_K4344040 (pEGFP-C1), BBa_K4344043 (pRK5 bb) and BBa_K4344044 (pRK5-HSV UL19 6xHis).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 15
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 15
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 15
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None