Generator

Part:BBa_K4202045:Experience

Designed by: Zhijian Yan, Zhuoyun Ren   Group: iGEM22_ZJU-China   (2022-10-03)
Revision as of 13:04, 12 October 2022 by Simplest (Talk | contribs) (→‎Method)


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The characterization experiment

Method

Measurement device construction

The sequence of this part was synthesis by Genscript and constructed to the pHY300PLK by In-Fusion clone.

Characterization

The transformant (Bacillus subtilis with pHY300PLK empty plasmid and kill switch) were inoculate to 2ml LB medium and cultured in the test tube overnight. The next day, the culture were inoculated into 2ml TB medium containing 1% sucrose (w/v) at the ratio of 1:100. After the OD600nm(L=1mm) reaches around 0.1, the OD600nm(L=1mm) were measured every 40minutes.

Result

User Reviews

UNIQ3cf4e02290c2ea5f-partinfo-00000000-QINU UNIQ3cf4e02290c2ea5f-partinfo-00000001-QINU