Composite

Part:BBa_K4273017

Designed by: Su Junzhe   Group: iGEM22_LINKS_China   (2022-09-30)
Revision as of 13:02, 12 October 2022 by Yu Jun (Talk | contribs)


pTDH3-DDGS-tTDH1-pPGK1-OMT-tPGK1

The first and second gene of gene clusters involved in biosynthesis of shinorine in cyanobacteria N. punctiforme.

encoding 2-demethyl 4-deoxygadusol synthase (DDGS) that converts sedoheptulose 7-phosphate (S7P) to 2-demethyl-4-deoxygadusol (DDG) and O-methyltransferase (O-MT) that converts 2-demethyl-4-deoxygadusol (DDG) to 4-deoxygadusol (4-DG)


Usage and Biology

Figure 1: Different combinations of AGL-ALAL genes found from different MAA-producing marine organisms. We used the strongest yeast constitutive promoter pTDH3 to express all AG-L genes, and the strong promoter pPGK1 to express all ALA-L genes, then inserted the 9 different combinations into 2μ plasmid vectors and transformed the plasmids into L3 to obtain L5 strain

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3270
    Illegal XhoI site found at 1922
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2168
    Illegal BsaI.rc site found at 3280


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