Part:BBa_K4129133

The fungal synthetic expression system with FunsTF70 and 6xLexO-Pmin

Fungal synthetic expression system using FunsTF70

In this fungal synthetic expression system (SES) FunsTF70 was constitutively expressed from PgpdA. The FunsTF70, which contains a LexA DNA-binding domain, would be able to bind to the LexO binding sites for the minimal promoter fused with LexO binding sites (6xLexO-Pmin). The efficiency of the FunsTF70 activation was measured by the reporter gene mCherry, which is expressed from the 6xLexO-Pmin.

For characterisation look up BBa_K4129114.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 4186
  Illegal XbaI site found at 6099
  Illegal PstI site found at 141
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 911
  Illegal NheI site found at 2986
  Illegal PstI site found at 141
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 145
  Illegal BglII site found at 3173
  Illegal BamHI site found at 2920
  Illegal BamHI site found at 5380
  Illegal XhoI site found at 701
  Illegal XhoI site found at 1083
  Illegal XhoI site found at 4050
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 4186
  Illegal XbaI site found at 6099
  Illegal PstI site found at 141
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 4186
  Illegal XbaI site found at 6099
  Illegal PstI site found at 141
  Illegal NgoMIV site found at 3078
  Illegal NgoMIV site found at 6016
  Illegal AgeI site found at 1290
  Illegal AgeI site found at 1438
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1117
  Illegal BsaI.rc site found at 5730
  Illegal SapI site found at 323