DNA

Part:BBa_K4438802

Designed by: Shreyas Samir Parkhie   Group: iGEM22_IISER-Tirupati_India   (2022-10-12)
Revision as of 12:48, 12 October 2022 by Shreyasparkhie17 (Talk | contribs)

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MN4_Target_1

MN4_Target_1(BBa_K4438802) is a single-stranded DNA sequence having 76 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. The 3’ end has ssDNA antisense T7 promoter sequence and the 5’ end has broccoli cDNA (antisense) sequence.


Usage and Biology

This part along with MN4_trigger_1-T7promoter-w/oPol(BBa_K4438801) and Aptamer_MN4(BBa_K4438800) can be used to detect different concentrations of quinine [1].

T--IISER-Tirupati India--mn4structure1.png



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Reinstein, O., Yoo, M., Han, C., Palmo, T., Beckham, S. A., Wilce, M. C., & Johnson, P. E. (2013). Quinine binding by the cocaine-binding aptamer. Thermodynamic and hydrodynamic analysis of high-affinity binding of an off-target ligand. Biochemistry, 52(48), 8652-8662.

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Categories
Parameters
None