Part:BBa_K4361105

pET-11a

A pET11a expression system in E. coli is popular because it combines a high protein yield with good regulation over the expression. The T7 RNA polymerase has a lacUV5 promoter that is Isopropyl 𝛃-D-1-thiogalactopyranoside (IPTG) inducible. With the addition of IPTG, the gene upstream of the T7 promoter can be transcribed. This part does not represent the full sequence of pET-11a, but rather the plasmid after digestion with BamHI as it allows for the insertion of a new DNA sequence at the cleavage site.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 316
  Illegal XbaI site found at 5594
  Illegal PstI site found at 1068
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 316
  Illegal NheI site found at 5639
  Illegal PstI site found at 1068
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 316
  Illegal BglII site found at 5528
  Illegal BamHI site found at 5672
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 316
  Illegal XbaI site found at 5594
  Illegal PstI site found at 1068
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 316
  Illegal XbaI site found at 5594
  Illegal PstI site found at 1068
  Illegal NgoMIV site found at 3394
  Illegal NgoMIV site found at 3748
  Illegal NgoMIV site found at 3908
  Illegal NgoMIV site found at 5496
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 1242
  Illegal SapI.rc site found at 2324