Part:BBa_K4438801

MN4_trigger_1-T7promoter-w/oPol

It is a single-stranded DNA sequence having 19 nucleotides (ssDNA sense T7 promoter strand). Figure 1A) shows the secondary structure and minimum free energy.

Usage and Biology

This part can bind to Aptamer_MN4 (BBa_K4438800) due to complementarity and disrupt its three-way junction. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. Quinine binds with cocaine-binding aptamer MN4 [1] and displaces the MN4_trigger_1-T7promoter-w/oPol(BBa_K4438801). This part has complete complementarity with MN4_Target_1(BBa_K4438802). T7 polymerase binds and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. All three parts can be used to detect different concentrations of quinine.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Reinstein, O., Yoo, M., Han, C., Palmo, T., Beckham, S. A., Wilce, M. C., & Johnson, P. E. (2013). Quinine binding by the cocaine-binding aptamer. Thermodynamic and hydrodynamic analysis of high-affinity binding of an off-target ligand. Biochemistry, 52(48), 8652-8662.