DNA

Part:BBa_K4438604

Designed by: Neha Adarsh   Group: iGEM22_IISER-Tirupati_India   (2022-10-11)
Revision as of 12:39, 12 October 2022 by Sejal (Talk | contribs) (→‎Usage and Biology)

T6_Target_2

T6_Target_2 is single-stranded DNA sequence having 97 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 5’ end has complementary nucleotides for part T6_Trigger_2_phi29 (BBa_K4438601) to bind. The middle region has the ssDNA sense T7 promoter sequence and the 3’ end has the ssDNA sense broccoli light-up aptamer sequence.


Usage and Biology

This part along with T6_Trigger_2_phi29 (BBa_K4438601) and Aptamer_T6 (BBa_K4438600) can used for sensing different concentrations of testosterone. https://parts.igem.org/File:T--IISER-Tirupati_India--T6_2.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 51
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 51
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 51
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 51
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Skouridou, V., Jauset-Rubio, M., Ballester, P., Bashammakh, A. S., El-Shahawi, M. S., Alyoubi, A. O., & O’Sullivan, C. K. (2017). Selection and characterization of DNA aptamers against the steroid testosterone. Microchimica Acta, 184(6), 1631-1639.

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