Part:BBa_K4509669:Design

Cell surface aphA with Cd2+ sensing promoter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1012
  

Design Notes

The metal sensitive promotor was chosen to ensure that acid phosphatase is expressed only in presence of cadmium. This would prevent the chassis demise due to high burden.

Source

<To be added>

References

1)Freudl, R., MacIntyre, S., Degen, M., & Henning, U. (1986). Cell surface exposure of the outer membrane protein OmpA of Escherichia coli K-12. Journal of molecular biology, 188(3), 491-494.

2)Gaddy, J. A., Tomaras, A. P., & Actis, L. A. (2009). The Acinetobacter baumannii 19606 OmpA protein plays a role in biofilm formation on abiotic surfaces and in the interaction of this pathogen with eukaryotic cells. Infection and immunity, 77(8), 3150-3160.

3)3.Kleiner‐Grote, G. R., Risse, J. M., & Friehs, K. (2018). Secretion of recombinant proteins from E. coli. Engineering in Life Sciences, 18(8), 532-550.

4)Sugawara, E., & Nikaido, H. (1992). Pore-forming activity of OmpA protein of Escherichia coli. Journal of Biological Chemistry, 267(4), 2507-2511.

5)5.Wang, Y. (2002). The function of OmpA in Escherichia coli. Biochemical and biophysical research communications, 292(2), 396-401.