Protein_Domain
Fok_a

Part:BBa_K243000

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-08)
Revision as of 12:06, 14 October 2009 by Timo (Talk | contribs) (→‎Sequence specific nuclease)

Protein domain (active) of the restriction endonuclease FokI

This part is used as the active domain of our universal restriction endonuclease. It cut DNA, when it fused with the inactive protein domain of our universal restriction endonuclease(BBa_K243001)and linked with specific oligos.


Sequence specific nuclease

This important development came when H.O. Smith, K.W. Wilcox, and T.J. Kelley, working at Johns Hopkins University in 1968, isolated and characterized the first restriction nuclease whose functioning depended on a specific DNA nucleotide sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 487


[edit]
Categories
//cds/enzyme/endonuclease/restriction
Parameters
None