Protein_Domain
Fok_a
Part:BBa_K243000
Designed by: Freiburg Bioware09 Group: iGEM09_Freiburg_bioware (2009-10-08)
Protein domain (active) of the restriction endonuclease FokI
This part is used as the active domain of our universal restriction endonuclease. It cut DNA, when it fused with the inactive protein domain of our universal restriction endonuclease(BBa_K243001)and linked with specific oligos.
Sequence specific nuclease
This important development came when H.O. Smith, K.W. Wilcox, and T.J. Kelley, working at Johns Hopkins University in 1968, isolated and characterized the first restriction nuclease]] whose functioning depended on a specific DNA nucleotide sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 487
[edit]
Categories
Parameters
//cds/enzyme/endonuclease/restriction
None |