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DNA
Part:BBa_K4169025
Designed by: Jie Min Group: iGEM22_HZAU-China (2022-10-01)
Revision as of 12:13, 12 October 2022 by Ruixuan Zhu (Talk | contribs)
The sequence between two reverse loxP sites can be flipped after accepting Cre's action
It is a 34bp DNA sequence consisting of two 13bp inverted repeats and an asymmetric 8bp spacer.The Cre recognizes the inverted repeats at each end of the loxP site and binds to form a dimer, which then binds to a dimer at the other loxP site to form a tetramer. If two loxP sites are located on the same DNA strand and in opposite directions, Cre recombinase mediates sequence flip between loxP.
Usage and Biology
asd
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |