DNA

Part:BBa_K4169025

Designed by: Jie Min   Group: iGEM22_HZAU-China   (2022-10-01)
Revision as of 12:13, 12 October 2022 by Ruixuan Zhu (Talk | contribs)


The sequence between two reverse loxP sites can be flipped after accepting Cre's action

It is a 34bp DNA sequence consisting of two 13bp inverted repeats and an asymmetric 8bp spacer.The Cre recognizes the inverted repeats at each end of the loxP site and binds to form a dimer, which then binds to a dimer at the other loxP site to form a tetramer. If two loxP sites are located on the same DNA strand and in opposite directions, Cre recombinase mediates sequence flip between loxP.

Usage and Biology

asd

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None