![](https://parts.igem.org/images/partbypart/icon_composite.png)
Part:BBa_K4509769:Design
Cell surface aphA with constitutive promoter
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 966
Design Notes
The constitutive promoter BBa_J23100 has been used since the strength of BBa_J23100 is more. This will facilitate overexpression of the protein on the cell surface.
References
Freudl, R., MacIntyre, S., Degen, M., & Henning, U. (1986). Cell surface exposure of the outer membrane protein OmpA of Escherichia coli K-12. Journal of molecular biology, 188(3), 491-494.
2.Gaddy, J. A., Tomaras, A. P., & Actis, L. A. (2009). The Acinetobacter baumannii 19606 OmpA protein plays a role in biofilm formation on abiotic surfaces and in the interaction of this pathogen with eukaryotic cells. Infection and immunity, 77(8), 3150-3160.
3.Kleiner‐Grote, G. R., Risse, J. M., & Friehs, K. (2018). Secretion of recombinant proteins from E. coli. Engineering in Life Sciences, 18(8), 532-550.
4.Sugawara, E., & Nikaido, H. (1992). Pore-forming activity of OmpA protein of Escherichia coli. Journal of Biological Chemistry, 267(4), 2507-2511.
5.Wang, Y. (2002). The function of OmpA in Escherichia coli. Biochemical and biophysical research communications, 292(2), 396-401.