Composite

Part:BBa_K4509769:Design

Designed by: MARIE JYOTSNA JAWAHAR, Rhea Harry William, Vibha Murali   Group: iGEM22_REC-CHENNAI   (2022-09-28)
Revision as of 12:05, 12 October 2022 by Marie JJ (Talk | contribs) (Source)


Cell surface aphA with constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 966


Design Notes

The constitutive promoter BBa_J23100 has been used since the strength of BBa_J23100 is more. This will facilitate overexpression of the protein on the cell surface.


References