Part:BBa_K4509469:Design
csgD and OmpR234 Dual Expression
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 877
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The strong promoter used in the construct is BBa_J23118. This promoter has been used so as to decrease the burden value or percentage reduction in growth rate.
References
1.Brombacher, E., Baratto, A., Dorel, C., & Landini, P. (2006). Gene expression regulation by the curli activator CsgD protein: modulation of cellulose biosynthesis and control of negative determinants for microbial adhesion. Journal of bacteriology, 188(6), 2027-2037.
2.Gerstel, U., Kolb, A., & Römling, U. (2006). Regulatory components at the csgD promoter–additional roles for OmpR and integration host factor and role of the 5′ untranslated region. FEMS microbiology letters, 261(1), 109-117.
3.Hammar, M. A. R. T. E. N., Bian, Z., & Normark, S. (1996). Nucleator-dependent intercellular assembly of adhesive curli organelles in Escherichia coli. Proceedings of the National Academy of Sciences, 93(13), 6562-6566.
4.https://2017.igem.org/Team:TAS_Taipei
5.Ogasawara, H., Yamada, K., Kori, A., Yamamoto, K., & Ishihama, A. (2010). Regulation of the Escherichia coli csgD promoter: interplay between five transcription factors. Microbiology, 156(8), 2470-2483.
6.Perni, S., Preedy, E. C., Landini, P., & Prokopovich, P. (2016). Influence of csgD and ompR on Nanomechanics, Adhesion Forces, and Curli Properties of E. coli. Langmuir, 32(31), 7965-7974.
7.Prigent-Combaret, C., Brombacher, E., Vidal, O., Ambert, A., Lejeune, P., Landini, P., & Dorel, C. (2001). Complex regulatory network controls initial adhesion and biofilm formation in Escherichia coli via regulation of the csgD gene. Journal of bacteriology, 183(24), 7213-7223.