Coding
Part:BBa_K4283010
Designed by: Lei Yingjie Group: iGEM22_SZ-SHD (2022-10-12)
Tumor antigen attached outer membrance vessicle(OMV-ClyA)
Outer membrane vesicles (OMVs) naturally secreted by E coli can work as delivery vehicles to carry display antigenic proteins on their surfaces and across intestinal epithelial barrier to where they can activate immune cells.
The design of the outer membrane vesicle (OMV) protein outlined in figure 1 contains three major structural domains: vesicle surface protein C terminus domain ClyA, cancer cell antigen Adpgk domain, and mouse IgG Fc fragment for the purpose of immune-enhancing and presenting antigen.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 72
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1713
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |