Coding

Part:BBa_K4283010

Designed by: Lei Yingjie   Group: iGEM22_SZ-SHD   (2022-10-12)
Revision as of 12:01, 12 October 2022 by Registry (Talk | contribs)

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Tumor antigen attached outer membrance vessicle(OMV-ClyA)

Outer membrane vesicles (OMVs) naturally secreted by E coli can work as delivery vehicles to carry display antigenic proteins on their surfaces and across intestinal epithelial barrier to where they can activate immune cells.

The design of the outer membrane vesicle (OMV) protein outlined in figure 1 contains three major structural domains: vesicle surface protein C terminus domain ClyA, cancer cell antigen Adpgk domain, and mouse IgG Fc fragment for the purpose of immune-enhancing and presenting antigen.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 72
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1713
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None