Part:BBa_K4509869:Design
HORSERADISH PEROXIDASE with constitutive promoter J23106
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 445
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 565
Illegal AgeI site found at 719
Illegal AgeI site found at 1022 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
By adding the constitutive promoter BBa_J23106, the burden of the part can be reduced.
Source
BBa_K1291071
References
Azevedo, A. M., Martins, V. C., Prazeres, D. M., Vojinovic, V., Cabral, J. M., & Fonseca, L. P. (2003). Horseradish peroxidase: a valuable tool in biotechnology. Biotechnology annual review, 9(3), 1387-2656.
Florea, M., Hagemann, H., Santosa, G., Abbott, J., Micklem, C. N., Spencer-Milnes, X., ... & Chughtai, H. (2016). Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain. Proceedings of the National Academy of Sciences, 113(24), E3431-E3440.
Francisco lucha, FernandoMartínez-García, CarlosLópez-García.1985.A new stabilizing agent for the tetramethyl benzidine (TMB) reaction product in the histochemical detection of horseradish peroxidase (HRP).Journal of Neuroscience Methods.13(2),0165-0270.
Frey, A., Meckelein, B., Externest, D., & Schmidt, M. A. (2000). A stable and highly sensitive 3, 3′, 5, 5′-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays. Journal of immunological methods, 233(1-2), 47-56.
Verlander, C. P. (1992). Detection of horseradish peroxidase by colourimetry. Non isotopic DNA probe techniques, 185-201.
https://parts.igem.org/Part:BBa_K1291071 Albers, S. C., Gallegos, V. A., & Peebles, C. A. M. (2015). Engineering of genetic control tools in Synechocystis sp. PCC 6803 using rational design techniques. Journal of Biotechnology, 216, 36–46.