DNA

Part:BBa_K4162029

Designed by: Weiwen Chen   Group: iGEM22_Fudan   (2022-10-06)
Revision as of 11:39, 12 October 2022 by Wave (Talk | contribs)


ribozyme + T7_RBS + BCMO + ribozyme + T7_RBS + ybbO-tdMCP-EGFP

Introduction

2022 Fudan

Coding sequence of BCMO and gene ybbO separated by hammerhead ribozyme. Gene ybbO is tagged with tdMCP and EGFP.

We use this biobrick to verify the expression ybbO in the presence of BCMO. Please check our measurement page for details.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2521
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2521
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2521
    Illegal AgeI site found at 1018
    Illegal AgeI site found at 1611
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2101
    Illegal BsaI site found at 2455


[edit]
Categories
Parameters
None