Composite

Part:BBa_K4204019:Design

Designed by: Jianfei Song   Group: iGEM22_BNDS_China   (2022-10-11)
Revision as of 11:38, 12 October 2022 by Jianfei Song (Talk | contribs) (→‎Design Notes)


IPTG induced GFP improved with ProQC system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 316
    Illegal NheI site found at 1116
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 978


Design Notes

The GFP could be replaced by any CDS of protein in interest. Since our team needs to extract the protein to prove its efficiency, the GFP is being replaced with LysPBC5, yielding BBa_K4204021 for characterization.

Source

The protein quality control system is from Yang et al's work [1]. Other components are from BBa_K653003. Assembled by BNDS_China 2022.

References

[1] Yang, J., Han, Y.H., Im, J. et al. Synthetic protein quality control to enhance full-length translation in bacteria. Nat Chem Biol 17, 421–427 (2021). https://doi.org/10.1038/s41589-021-00736-3