Composite

Part:BBa_K180002

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-14)
Revision as of 08:18, 14 October 2009 by Registry (Talk | contribs)

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tac pR testing plasmid (GFP)

PTAC promoter followed by an RBS, green fluorescent protein and stop codon, for testing the effectiveness of the PTAC hybrid promoter designed by iGEM2009 Team:Southampton.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2117


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
controltrp, lac
directionforward
emission511 nm
emit511
excitation501 nm
excite501
lum
n/atac pR testing plasmid (GFP)
proteinGFPmut3b
tagNone