Part:BBa_K4390110

L2NC tag expression (control)

Usage and Biology

A truncated version of the L2 ribosomal protein from E. coli, for fusion to N-terminal of a protein using JUMP assembly (N-terminal 1-60 and C-terminal 203-273 amino acids of L2 [silica-binding regions of L2 only] 130 amino acids). This tag contains just the N and C-terminal regions of L2 which were shown to have silica binding capacity, allowing the use of a smaller tag without compromising on binding affinity. From literature, the dissociation constant between L2NC silica tag and silica beads is 1.7nM. The N-terminal L2NC silica tag is inspired by 2021 Edinburgh OG teams (BBa_K3946002).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 108
  
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

Kim S, Joo K, Jo B, Cha H. Stability-Controllable Self-Immobilization of Carbonic Anhydrase Fused with a Silica-Binding Tag onto Diatom Biosilica for Enzymatic CO2 Capture and Utilization. ACS Applied Materials & Interfaces. 2020;12(24):27055-27063.