Composite

Part:BBa_K4390092

Designed by: Zhongyi Liang   Group: iGEM22_Edinburgh-UHAS_Ghana   (2022-09-14)
Revision as of 11:26, 12 October 2022 by Xianliang (Talk | contribs) (→‎Usage and Biology)


His-tagged WT RolA


Usage and Biology

The wild-type RolA with 6His-tag for Nickel affinity column purification.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 169
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 161


[edit]
Categories
Parameters
None