Regulatory

Part:BBa_K4204015:Design

Designed by: Jianfei Song   Group: iGEM22_BNDS_China   (2022-10-08)
Revision as of 11:16, 12 October 2022 by Jianfei Song (Talk | contribs) (→‎References)

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pJEx-D + RBS of crystal violet induction system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 89
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The eilOc (crystal violet regulator) has a palindromic sequence. It enables the mRNA's 5'end to fold and stable it. However, this also create multiple primer binding site at that location, making appropriate PCR hard to perform.


Source

From research done by Ruegg et al [1]. Synthesized by twist bioscience.

References

[1] Ruegg, T. L., Pereira, J. H., Chen, J. C., DeGiovanni, A., Novichkov, P., Mutalik, V. K., Tomaleri, G. P., Singer, S. W., Hillson, N. J., Simmons, B. A., Adams, P. D., & Thelen, M. P. (2018). Jungle Express is a versatile repressor system for tight transcriptional control. Nature Communications, 9(1). https://doi.org/10.1038/s41467-018-05857-3