Coding

Part:BBa_K4169024

Designed by: Jie Min   Group: iGEM22_HZAU-China   (2022-10-01)
Revision as of 11:09, 12 October 2022 by Ruixuan Zhu (Talk | contribs)


produce Cre enzyme

Two loxP sites are on the same DNA strand and in opposite directions. And after expressing, it'll produce Cyclic recombinase which mediates sequence flip between loxPs.

Usage and Biology

When we express the Cre which recognizes the reverse repeat sequence at both ends of the loxP site and binds to form a dimer, which then binds to a dimer at another loxP site to form a tetramer.This allows us to have our engineered bacteria flip the gene between the two reverse loxP sites. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 356
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None