Composite

Part:BBa_K4229061:Design

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-30)
Revision as of 11:07, 12 October 2022 by NikitaEdel (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


sfGFP with targetpeptide for encapsulins regulated by T7 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 107


Design Notes

none


Source

m.Xhantus is the original organism, but we synthesized the codon-optimized version of the encapsulin.

References

[1] J. Fontana et al., “Phage capsid-like structure of Myxococcus xanthus encapsulin, a protein shell that stores iron,” Microsc. Microanal., vol. 20, no. 3, pp. 1244–1245, 2014, doi: 10.1017/S1431927614007958.

[2] F. Johnsson, J. Kjärstad, and J. Rootzén, “The threat to climate change mitigation posed by the abundance of fossil fuels,” Clim. Policy, vol. 19, no. 2, pp. 258–274, 2019, doi: 10.1080/14693062.2018.1483885.