Composite

Part:BBa_K4119024

Designed by: Chengtai Yin   Group: iGEM22_NJTech_China   (2022-09-30)
Revision as of 11:04, 12 October 2022 by JiangTao1103 (Talk | contribs)


Pvgb-double promoter->Bs2->Cpa fdx terminator

it's Pvgb-double promoter->Bs2->Cpa fdx terminator


Document

Results:

The inhibition effect of the improved promoter(P2vgb) was stronger than that of the control group(Pvgb) under aerobic conditions. Under the induction of microaerobic conditions, the improved promoter(P2vgb) behaves similarly to the control group(Pvgb) under aerobic conditions. This suggests that tandem promoters improve protein expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 485
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 485
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 485
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 485
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None