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Part:BBa_K4501020:Experience

Designed by: Pau Marín Escudero   Group: iGEM22_Barcelona_UB   (2022-09-22)
Revision as of 10:58, 12 October 2022 by Paumarin99 (Talk | contribs)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4501020

We demonstrated the downregulation of myc by transiently transfecting (by electroporation) these shRNA in a expression cassette on Ramos and HEK293T cell lines. qPCR from total RNA extraction shows as high as almost 2-fold decrease in myc expression when treatment with shRNA.

ShRNA qPCR complete.jpg Figure 1. (A) Results of qPCR for Ramos and HEK293T cell line. (B) GFP expression of the cell lines transfected with the constructions.

User Reviews

UNIQ290c5e26952a9851-partinfo-00000000-QINU UNIQ290c5e26952a9851-partinfo-00000001-QINU