Coding

Part:BBa_K4244031

Designed by: Arthur Vancolen   Group: iGEM22_Wageningen_UR   (2022-10-08)
Revision as of 10:51, 12 October 2022 by Sarah99 (Talk | contribs)

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dSpyCas9 fused to a luciferase protein

dSpyCas9 fused to a luciferase protein to measure the amount of dCas9 at a specific time.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1340
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1340
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1340
    Illegal BamHI site found at 3378
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1340
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1340
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None