Reporter

Part:BBa_K4244025

Designed by: Arthur Vancolen   Group: iGEM22_Wageningen_UR   (2022-10-08)
Revision as of 10:50, 12 October 2022 by Sarah99 (Talk | contribs)

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Three-step inducible system with mRFP

This plasmid contains one component out of three of the three-step inducible system. Here the behaviour of the system with different lengths of the B1 spacer can be tested by measuring the mRFP fluorescence. This construct consists of mRFP coding sequence with a dSpyCas9 target site in between the promoters and coding sequence of this protein. Additionally, an introduction template for sgRNA spacers was introduced. This template contains two type IIS restriction sides, this way different length B1 spacers can be easily introduced and tested.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 151
    Illegal XbaI site found at 157
    Illegal SpeI site found at 30
    Illegal PstI site found at 169
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 151
    Illegal NheI site found at 7
    Illegal NheI site found at 307
    Illegal SpeI site found at 30
    Illegal PstI site found at 169
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 151
    Illegal BglII site found at 181
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 151
    Illegal XbaI site found at 157
    Illegal SpeI site found at 30
    Illegal PstI site found at 169
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 151
    Illegal XbaI site found at 157
    Illegal SpeI site found at 30
    Illegal PstI site found at 169
    Illegal AgeI site found at 908
    Illegal AgeI site found at 1020
  • 1000
    COMPATIBLE WITH RFC[1000]


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