Regulatory

Part:BBa_K4193004

Designed by: Rui Shi   Group: iGEM22_OUC-China   (2022-09-29)
Revision as of 10:44, 12 October 2022 by NTSay (Talk | contribs)


SSRE promoter

Quorum sensing system related promoter

Usage and Biology

Cytokinin isopentenyladenine (IP), as quorum sensing molecule, is the product of ATP catalyzed by AtlPT4. When the signal molecule IP reaches a certain concentration, it activates the SSRE promoter and increases the expression of production gene controlled by SSRE promoter in our experiment, to better characterize SSRE promoter and quorum sensing circuit, we put eGFP gene at the downstream of SSRE promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 239
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 239
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 239
    Illegal NgoMIV site found at 6
    Illegal NgoMIV site found at 46
    Illegal NgoMIV site found at 86
    Illegal NgoMIV site found at 126
    Illegal AgeI site found at 16
    Illegal AgeI site found at 56
    Illegal AgeI site found at 96
    Illegal AgeI site found at 136
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None