![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4201021
Designed by: David Birkhaeuser Group: iGEM22_CU-Boulder (2022-10-12)
CrtE_cytoTDS2-MBP_T5αOH_RUBY
This construct contains four main segments: CrtE, cytoTDS2-MBP, T5αOH, and RUBY. Each segment encodes for a particular gene(s) of interest, and is enclosed by an upstream Gmubi promoter and downstream AtHSP terminator. CrtE encodes for a GGPP synthase that converts DMAPP into GGPP1, a precursor in the paclitaxel pathway. Taxadiene synthase II, an enzyme encoded by cytoTDS2, then converts the GGPP into taxadiene. This taxadiene is used by T5αOH to synthesize Taxadiene-5α-ol, the next molecule in the paclitaxel pathway.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 8103
Illegal BglII site found at 790
Illegal BglII site found at 1153
Illegal BglII site found at 2021
Illegal BglII site found at 2956
Illegal BglII site found at 6827
Illegal BglII site found at 7762
Illegal BglII site found at 8719
Illegal BamHI site found at 13070 - 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI site found at 948
Illegal BsaI site found at 1887
Illegal BsaI site found at 2167
Illegal BsaI site found at 3114
Illegal BsaI site found at 6693
Illegal BsaI site found at 6973
Illegal BsaI.rc site found at 934
Illegal BsaI.rc site found at 1873
Illegal BsaI.rc site found at 2153
Illegal BsaI.rc site found at 3100
Illegal BsaI.rc site found at 6679
Illegal BsaI.rc site found at 6959
Illegal BsaI.rc site found at 7906
[edit]
Categories
Parameters
None |