Composite

Part:BBa_K4201021

Designed by: David Birkhaeuser   Group: iGEM22_CU-Boulder   (2022-10-12)
Revision as of 10:41, 12 October 2022 by Dabirk (Talk | contribs)


CrtE_cytoTDS2-MBP_T5αOH_RUBY

This construct contains four main segments: CrtE, cytoTDS2-MBP, T5αOH, and RUBY. Each segment encodes for a particular gene(s) of interest, and is enclosed by an upstream Gmubi promoter and downstream AtHSP terminator. CrtE encodes for a GGPP synthase that converts DMAPP into GGPP1, a precursor in the paclitaxel pathway. Taxadiene synthase II, an enzyme encoded by cytoTDS2, then converts the GGPP into taxadiene. This taxadiene is used by T5αOH to synthesize Taxadiene-5α-ol, the next molecule in the paclitaxel pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 8103
    Illegal BglII site found at 790
    Illegal BglII site found at 1153
    Illegal BglII site found at 2021
    Illegal BglII site found at 2956
    Illegal BglII site found at 6827
    Illegal BglII site found at 7762
    Illegal BglII site found at 8719
    Illegal BamHI site found at 13070
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 948
    Illegal BsaI site found at 1887
    Illegal BsaI site found at 2167
    Illegal BsaI site found at 3114
    Illegal BsaI site found at 6693
    Illegal BsaI site found at 6973
    Illegal BsaI.rc site found at 934
    Illegal BsaI.rc site found at 1873
    Illegal BsaI.rc site found at 2153
    Illegal BsaI.rc site found at 3100
    Illegal BsaI.rc site found at 6679
    Illegal BsaI.rc site found at 6959
    Illegal BsaI.rc site found at 7906


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Categories
Parameters
None