Coding

Part:BBa_K4169024

Designed by: Jie Min   Group: iGEM22_HZAU-China   (2022-10-01)
Revision as of 10:38, 12 October 2022 by Ruixuan Zhu (Talk | contribs)


produce Cre enzyme

Two loxP sites are on the same DNA strand and in opposite directions. And after expressing, it'll produce Cyclic recombinase which mediates sequence flip between loxPs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 356
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None