Plasmid

Part:BBa_K4181001

Designed by: Junzhi Yan   Group: iGEM22_SJTU-BioX-Shanghai   (2022-09-18)
Revision as of 10:36, 12 October 2022 by JunzhiYan (Talk | contribs)

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A transforming plasmid of Bacillus subtilis

If you want to insert a target gene into the genome of Bacillus subtilis, you can insert it between two amyE sites in the plasmid, and then you just transform the plasmid into Bacillus subtilis, and you can do that.This is a plasmid that inserts a target gene into the chromosome gene of Bacillus subtilis.

Plasmid profiles

2022-SJTU-Biox-Shanghai-11.png

Figure 1.Pht01.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 653
    Illegal XbaI site found at 815
    Illegal XbaI site found at 846
    Illegal XbaI site found at 5395
    Illegal SpeI site found at 5491
    Illegal PstI site found at 659
    Illegal PstI site found at 3650
    Illegal PstI site found at 6846
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 653
    Illegal SpeI site found at 5491
    Illegal PstI site found at 659
    Illegal PstI site found at 3650
    Illegal PstI site found at 6846
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 653
    Illegal BamHI site found at 635
    Illegal XhoI site found at 5745
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 653
    Illegal XbaI site found at 815
    Illegal XbaI site found at 846
    Illegal XbaI site found at 5395
    Illegal SpeI site found at 5491
    Illegal PstI site found at 659
    Illegal PstI site found at 3650
    Illegal PstI site found at 6846
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 653
    Illegal XbaI site found at 815
    Illegal XbaI site found at 846
    Illegal XbaI site found at 5395
    Illegal SpeI site found at 5491
    Illegal PstI site found at 659
    Illegal PstI site found at 3650
    Illegal PstI site found at 6846
    Illegal NgoMIV site found at 5709
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 6672
    Illegal SapI.rc site found at 1433


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