Coding

Part:BBa_K4229029

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-29)
Revision as of 10:27, 12 October 2022 by Johanna G (Talk | contribs)

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BMC-H Protein

The H-protein assembles the basic shell of the wiffelballs (minimal-wiffelball: BBa_K4229047/ full-wiffelball: BBa_K4229049) that we used as compartmentalisation system. It was desinged after the HO-shell found in Haliangium orchaceum.

A:Shows the original HO-Shell found in Haliangium orchaceum. B: Shows the full wiffleball made with H-/T1-/T2-/T3-protein. C: minimal wiffleball made just of the H- and T1-protein.























The plasmid containing this protein was kindly sent to us by the Kerfeld Group after we found this very interesting paper:

H. Kirst and C. A. Kerfeld, “Bacterial microcompartments: Catalysis-enhancing metabolic modules for next-generation metabolic and biomedical engineering,” BMC Biol., vol. 17, no. 1, pp. 1–11, 2019, DOI: 10.1186/s12915-019-0691-z.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None