![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4201019
Designed by: Lindsey Neukirch Group: iGEM22_CU-Boulder (2022-10-12)
crtE-cytoTDS-MBP_T5αOH_RUBY
This composite part is made using a Kan BsaI backbone. The B/W selection was excised during Golden Gate synthesis and replaced with a sequence containing CrtE-cytoTDS-MBP, T5αOH, and RUBY genes. Each inserted gene is flanked by a Gmubi promoter and AtHSP terminator. This construct was made to be transfected into agrobacterium and then into Glycine max. The Crte-cytoTDS-MBP is a fusion protein that codes for the first two enzymes in the paclitaxel biosynthesis pathway, a GGPP synthase1 and taxadiene synthase2. The T5αOH encodes for the third step in the biosynthesis of paclitaxel3. The RUBY reporter was added to visualize the construct when integrated into the G. max genome4
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6826
Illegal PstI site found at 994
Illegal PstI site found at 4792
Illegal PstI site found at 5632
Illegal PstI site found at 8365
Illegal PstI site found at 13549 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6826
Illegal PstI site found at 994
Illegal PstI site found at 4792
Illegal PstI site found at 5632
Illegal PstI site found at 8365
Illegal PstI site found at 13549 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6826
Illegal BglII site found at 790
Illegal BglII site found at 1610
Illegal BglII site found at 3148
Illegal BglII site found at 4195
Illegal BglII site found at 5566
Illegal BglII site found at 6493
Illegal BglII site found at 7442
Illegal BamHI site found at 5246
Illegal BamHI site found at 11769
Illegal XhoI site found at 2571 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6826
Illegal PstI site found at 994
Illegal PstI site found at 4792
Illegal PstI site found at 5632
Illegal PstI site found at 8365
Illegal PstI site found at 13549 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6826
Illegal PstI site found at 994
Illegal PstI site found at 4792
Illegal PstI site found at 5632
Illegal PstI site found at 8365
Illegal PstI site found at 13549
Illegal NgoMIV site found at 6975
Illegal NgoMIV site found at 9705
Illegal NgoMIV site found at 10284
Illegal NgoMIV site found at 11997
Illegal AgeI site found at 937
Illegal AgeI site found at 2313
Illegal AgeI site found at 5429
Illegal AgeI site found at 5701
Illegal AgeI site found at 6640
Illegal AgeI site found at 8162
Illegal AgeI site found at 8434 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI site found at 940
Illegal BsaI site found at 5432
Illegal BsaI site found at 5704
Illegal BsaI site found at 6643
Illegal BsaI site found at 8165
Illegal BsaI site found at 8437
Illegal BsaI.rc site found at 934
Illegal BsaI.rc site found at 5426
Illegal BsaI.rc site found at 5698
Illegal BsaI.rc site found at 6637
Illegal BsaI.rc site found at 8159
Illegal BsaI.rc site found at 8431
Illegal BsaI.rc site found at 9370
Illegal SapI.rc site found at 3369
Illegal SapI.rc site found at 3575
[edit]
Categories
Parameters
None |